In this photo of areolar connective tissue, nuclei of cells are stained but the cytoplasm is pale and not distinguishable. Connective tissue fills the spaces between organs and tissues, and provides structural and metabolic support for other tissues and organs. (CT10). In connective tissues cells typically account for only a small fraction of the tissue volume. Questions are provided for the most important slides of this chapter. As they have a phagocytic function, removing pathogens and cell debris, macrophages usually contain abundant primary and secondary lysosomes. Such networks are widespread among different tissues and form supporting frameworks in the liver, lymphoid organs, capillary endothelia, and muscle fibers. Oct 14, 2019 - Explore GABRIELLA RUESCHHOFF's board "Connective Tissue" on Pinterest. These are fibroblasts View Image (as opposed to clusters of similar appearing elongate nuclei that are usually smooth muscle cells or Schwann cells in a nerve that you will learn to recognize soon). Slide 160 (stomach, PAS & Azure II) View Virtual Slide. Where are the nuclei of the fat cells? Slide 26 (lymph node, H&E) View Virtual Slide. You need to get 100% to score the 7 points available. Protein secreting cells have a basophilic cytoplasm because they are full of rough ER, which stains with hematoxylin, a basic dye. Plasma cells may also be foundwithin the connective tissue of many of the glands that secrete into these regions. When fat cells have accumulated in such abundance that they crowd out or replace cellular and fibrous elements, the accumulation is termed adipose tissue. Areolar Connective Tissue For connective tissue stains – Van Gieson stains and variants. A collagen fibril -the brackets enclose a single collagen FIBRIL, which is made of 1000s of tropocollagen units attached in a head-to-tail arrangement. It is made up of type II collagen and is located in the auricle of the ear and the epiglottis. Fibroblasts are large, flat, elongated (spindle-shaped) cells possessing processes extending out from the ends of the cell body. Dense, regular collagenous. Slide 88 is stained with H&E where the concentric rings of elastic lamellae are intensely stained with eosin giving a glassy red appearance View Image (one of the few places where elastin is easy to recognize in H&E sections). This region is also a loose, irregular connective tissue but can be so extensively infiltrated by white blood cells and plasma cells that the supporting fibers and ground substance are obscured. (CT8). The fine collagenous network that provides support in the bone marrow, lymphatic organs, around individual smooth muscle cells, and beneath most epithelia is composed mainly of Type III collagen. Start studying Lab 4: Connective Tissue Microscope Slides. Location. In the nucleus, areas of euchromatin and heterochromatin can easily be identified. Be able to describe the functions of cells commonly found in connective tissue and identify them. Adipose or fat tissue has no visible fibers. (CT9) Test your ability to identify different organelles at this magnification! Attempt and answer these flashcards quizzes easily and have a smooth … Although collagen fibers mostly fill the view, there are numerous elastic fibers, which provide the elasticity essential for the function of the tissue. ), note how a network of fine black fibrils is present in this same tissue following silver staining. Slide 40 (trachea, H&E) View Virtual Slide. Neutrophils generally enter tissues in large numbers only in response to a disease stimulus. This quiz only covers material that one would be expected to discuss in a histology laboratory. What functions does this type of connective tissue play to support the epithelium? The English word "tissue" derives from the French word "tissue", meaning that … These tissues have two principal components, an extracellular matrix and a variety of support cells. Even though we try to set up rigid categories (e.g. Slide 106 and 112 have bits of well preserved flexor tendon at the top of the section (the tissue at the very top of slide 112 is actually skeletal muscle -which you'll study in the next lab; the tendon is just below it). Look in the lamina propria amongst the plasma cells and you will find neutrophils that have emigrated from the bloodstream into the tissue space as part of the immune response. Many of the free cells in these medullary sinuses cannot be identified; however, the large rounded cells, with eccentrically placed, vesicular nuclei are the ones you should try to find. What was present in the "empty" looking intercellular space? They are especially common along smaller blood vessels. The function of white fat is to serve as an energy source and thermal insulator. Remember that each fat cell is enclosed by a thin basal lamina (Unfortunately, in these examples you can’t see the basal lamina). Observe the large amount of rough endoplasmic reticulum (ER) in these cells. Histology of Connective Tissues View Online Download for PC for Mobile Contents General Characteristics Structure Functions Matrix You can see one large lipid droplet in the cytoplasm of each cell. It develops from the mesoderm. The Golgi complex in these particular plasma cells is usually in the form of a fine crescent adjacent to the nucleus and it takes some practice to recognize. slide 36 (Aorta, aldehyde fuchsin) View Virtual Slide. Mast cells are granulated cells typically found in connective tissue. Variations in the … What is their function. In this area note the irregular, wavy collagen fibers arranged singly or in small groups. Fibroblast, the principal active cell of connective tissue. However, deeper in the dermis, the collagen and elastic fibers are much thicker. Leukocytes - white blood cells; have nucleus; dyed purple to distinguish under microscope.) Cartilage is a specialized form of connective tissue produced by differentiated fibroblast-like cells called chondrocytes. 7 Types of Connective Tissue - Microscope Slides learn by taking a quiz; Online quiz to learn 7 Types of Connective Tissue - Microscope Slides; Your Skills & Rank. Connective tissue generally consists of a considerable amount of extracellular material called matrix. Note at high power that fibrils or fibers of any type cannot be readily observed. Correct answer 4. Lacunae are separated from one another as a result of the secretory activity of the chondrocytes. A final, special connective tissue is vascular or blood tissue. It is significantly weaker in individuals with Marfan syndrome. Macrophages phagocytose foreign material in the connective tissue layer and also play an important role as antigen presenting cells, a function that you will learn more about in Immunobiology. These fibers are often organized into lamellar sheets, as in the walls of arteries. These two components will be the focus of this lab. Dense regular connective tissue 400X Tendon In this image a fibroblast nucleus (fb nuc) is labeled, but you can see other nuclei once you know what to look for. Health Information Technology & Services. The ribosomes attached to rough ER are associated with both ribosomal and messenger RNA - these nucleic acids carry a net negative charge and bind basic dyes (which are positively charged). 4 Connective tissues Most of the body is made of connective tissues. NOTE:  Slide 250 illustrates a point about the limits of classification schemes. = acidophilic) region adjacent to the cell nucleus. The granules are often so dark that they obscure the nucleus. Look for plasma cells within the lamina propria of slide 29 View Image. The fibrous components of loose areolar connective tissue, when viewed with the light microscope, appear as colourless strands of varying diameter running in all directions, and, if not under tension, these have a slightly undulant course. The structure indicated is an elastic fiber, which stains purple/black with aldehyde fuchsin and would be significantly weaker in individuals with Marfan syndrome. Name the cell type and its major product. Learn the terms, keywords, vocabulary, and much more about Microscopic Images of Tissues with our flashcards quizzes. Neutrophils and macrophages are also present and both are discussed below. Unlike the tissue of the aorta which would be classified as "regular," these tissues are obviously irregular but they're mentioned here for the purpose of illustrating how aggregates of elastic fibers appear in H&E-stained sections. White fat cells or adipocytes are specialized for the storage of triglyceride, and occur singly or in small groups scattered throughout the loose connective tissue. & Mass. 25 Connective Tissue - Fibroblast View Virtual EM Slide The tissue sections after fixation and wax embedding are typically cut into two to five micron thin slices with a microtome before staining and transfer to a glass slide for examination with a light microscope. This connective tissue is the only liquid tissue in the body. In the central nervous system, the three outer membranes that envelop the brain and spinal cord are … Identify the tissue depicted in the below micrograph. Loose (areolar connective tissue) is the most abundant form of collagenous connective tissue. This tissue section stained with aldehyde fuchsin and eosin. Because of their nuclear morphology, they are frequently also called “polymorphonuclear leukocytes” (aka "PMNs" or “polys”). This electron micrograph shows a typical secretory cell, a plasma cell, which secretes immunoglobulin protein. Neutrophils can be identified by their granular cytoplasm and their multilobular, condensed nuclei. Now with slide 28 (make sure your slide is stained with silver; it should say “Ag” on the label! Brown fat cells would have several small lipid droplets all of which would be roughly the same size. You can't see the rest of the fibroblast cell because it stains the same color as the collagen fibers. 29 Tendon - Longitudinal section View Virtual EM Slide Two other locations where elastic fibers can be readily seen in H&E sections are in the lamina propria of the pharynx (slide 152 View Image ) and the trachea (slide 40 View Image ). Is this an indication of an active or inactive cell? Elastic fibers contain the protein elastin, which co-polymerizes with the protein fibrillin. Game Points. Collagenous connective tissue is divided into two types, based upon the ratio of collagen fibers to ground substance. Be able to recognize a basement membrane (or basal lamina) in sections or micrographs where the structure is conspicuously present and understand its functions. Be able to recognize interstitial (fibrillar) collagens and elastic fibers at the light and electron microscopic levels. These cells make a large amount of protein that they secrete to build the connective tissue layer. Now look at the region immediately underneath the intestinal epithelium, which is called the lamina propria View Image . These are reticular fibers, found in skin, muscle and blood vessels. Fibroblasts are by far the most common native cell type of connective tissue. your own Pins on Pinterest It consists of fiber bundles that appear dark under the microscope and has many disc-shaped cells interspersed throughout that are white in color. These are termed reticular (or rarely, argyrophilic= "silver loving") fibers. Mast cells are most abundant in the connective tissue associated with the lining of the digestive and respiratory systems, and your collection just so happens to contain a tissue section from the stomach that has been stained with PAS and Azure II. 28 Collagen and Elastin - Cross section of Chorda Tendinea View Virtual EM Slide The antibody binds mast cells and waits for a second exposure to whatever it happens to be responsive to (an allergen). Look for elongated nuclei, usually solitary, from which a modest amount of tapered cytoplasm extends from either one or both poles of the nucleus. It is comprised primarily of type I collagen. The degranulation of these cells is responsible for triggering type I, immediate hypersensitivity reactions. Slide 106 (plantar skin and tendon, H&E) View Virtual Slide, Slide 112 (plantar skin and tendon, H&E) View Virtual Slide. Observe the branching nature of the elastic fiber and the "mantle" of elastic microfibrils. Find the Golgi complex, a pale or slightly eosinophilic (=eosin "loving", an area rich in membranes containing basic amino acids, syn. Type I reactions, also called anaphylactic reactions, are something you will learn about while studying the immune system later on, but for now, here is a quick explanation. Collagenous fibers consist of types I, II, or III collagen and are present in all types of connective tissue. You should note that the cytoplasm is quite basophilic (i.e. It is significantly weaker in individuals with type IV Ehlers-Danlos syndrome. Ground substance is an aqueous gel of glycoproteins and proteoglycans that occupies the space between cellular and fibrillar elements of the connective tissue. The luminal surface of the intestine is made up of a simple columnar epithelium. Note how the diameter of the fibers varies with location. Hem. Be sure you recognise favourable sections of the nucleas, mitochondria, and rough ER. Microscope at 400X. 3. Dense connective tissue is full of fibroblasts. They phagocytose cell debris and/or foreign material. "loose" versus "dense" connective tissue), sometimes it is not always possible to classify connective tissue in a given section; it may be a little loose, a little dense, a little fatty, etc. associated with allergies and other type I reactions. Stain results: Septa: Red: ... Electron Microscopy Sciences specializes in the manufacturing, preparation and distribution of the highest quality laboratory chemicals and microscopy supplies and equipment for electron microscopy, light microscopy, and histology. Non-striated (involuntary) muscle E 5 Striated muscle, showing striations and nuclei LS 5 Adipose tissue, section 5 Pseudo-stratified ciliated: epithelium TS 5 Areolar tissue, stained for fibres and cells E 5 Spinal cord TS 5 Compact bone, for Haversian Canals and lamellae 5 Cardiac muscle showing the nuclei, striations and intercalated discs LS 5 When the IgE binds an appropriate molecule, it will trigger degranulation of the mast cell and the vasodilation, congestion, bronchiolar constriction, wheezing, etc. Connective tissue is one of the four basic types of animal tissue, along with epithelial tissue, muscle tissue, and nervous tissue. However, on a microscope slide you may see open spaces around the connective tissues. Typical specimens in pathology are colon, kidney, pancreas, cervix, lung, breast, prostate, or connective tissue. With low power, locate the medulla (the interior) of the lymph node. Be sure you look at both the H&E and Masson trichrome-stained slides as they provide an excellent opportunity to see how collagen stains in connective tissue when either stain is applied. In this image of an artery, What type of fibers are stained darkly? Identify the correct statement about the structure that is indicated by the black arrow. Usually there is more extracellular matrix than cellular material. Connective tissue consists of cells separated by varying amounts of extracellular substance. In the region immediately beneath the epidermis you can see how the elastic fibers are interconnected forming an elaborate, delicate net of fibers View Image amongst thin strands of collagen. Fat cell nuclei are on the periphery of the cell and may or may not be in the plane of section. The cell nucleus is flat and oval. Thus, what distinguishes the different connective tissues is the type of matrix. In this section, the fibers clearly predominate. These slides are examples of regularly arranged sheets (lamellae) of elastin. Access to the supplemental resources for this session is password-protected and restricted to University of Michigan students. The fibroblast synthesizes the collagen and ground substance of the extracellular matrix. Chapter 3 - Connective Tissue. They phagocytose cell debris and/or foreign material. It occurs in small, elongated bundles separated by regions that contain ground substance. 1. This generates heat. Tissue macrophages can be found in many different organs. Describe the structural organization of the fibers in the extracellular matrix and the cells residing within connective tissue, Distinguish loose and dense connective tissue using the light microscope, Describe the structure and function of cartilage. 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